Biorobot ez1 user manual

Papers from cigarette butts or 3 paper discs per sample were spotted with 10 ng DNA per sample. Solid materials were removed from the remaining half of the samples, which were then processed using the EZ1 DNA Investigator Kit with the standard trace protocol Trace.

This not only allows efficient DNA purification from dilute samples with low concentrations of DNA, such as diffuse stains, but also enables purification from samples that require larger volumes for thorough lysis. The ability to process larger sample volumes — with the same elution volume as the standard trace protocol — enables higher yields of more concentrated DNA for greater sensitivity in downstream applications see Higher yields of concentrated DNA with the large-volume protocol.

Additional EZ1 Cards may be purchased at any time to increase the application range of EZ1 instruments. Simply insert the EZ1 Card with the desired protocol into the card slot and start the EZ1 instrument.

Operation of the BioRobot EZ1 is so easy that no additional training other than the initial demo is needed. Operating the instrument requires only three major steps:. The normalization protocol on the EZ1 DNA Investigator Card is useful for routine, standardized analyses of reference samples, such as blood or buccal swabs, where sample-to-sample variability can result in widely varying DNA yields.

The normalization protocol eliminates the need for yield quantification prior to analysis. It limits DNA yields uniformly to ng. However, please note that the normalization protocol should not be used if the DNA amount in the sample is very limited. Protocols for nucleic acid purification are supplied on pre-programmed EZ1 Protocol Cards. These protocols provide both on-screen instructions for the operator and operating commands for the workstation. The range of kits and protocols for the EZ1 is continuously expanding to allow nucleic acid purification from a wide range of sample types.

Please browse our Supplementary Protocol Section to find specialized EZ1 protocols for a large variety of clinical sample types, in addition to the standard sample types covered in our EZ1 handbooks.

Loading of sample tubes, elution tubes, filter-tips, and Reagent Cartridges onto the worktable of the BioRobot EZ1 is intuitive, easy and quick. Details about worktable setup are provided with each new protocol, and are also displayed in the liquid-crystal display LCD of the control panel when you start setup of the worktable. During operation of the instrument, the piercing unit, a row of 6 metal spikes located behind the tip adapters, will puncture the foil of the cartridges, thereby exposing the reagents.

Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach. Reorder now!

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Log Out. Show More. Peak heights for 8 peaks were normalized within each experiment. Higher yields of concentrated DNA with the large-volume protocol. Improved performance in STR analysis. Resources Supplementary Protocols 0. User-Developed Protocols 0. Quick-Start Protocols 0. Validation Reports 0. Application Notes 0. Kit Handbooks 0. Additional Resources 0.